1st week General information

2nd week - recording techniques - recording devices: kimograph, computer - methods of stimulation: thermal, chemical, electrical, types of electrodes - video - Dissection of the frog: - anaesthesia - decapitation - lesioning of the spinal cord - movements of cilia (frog's palate) - heart - organs in the abdomen - m. sartorius preparation - m. gastrocnemius nerve-muscle preparation - solutions used in physiological experiments

4th week Principles of blood tests: - the microscope in hematological tests - sterilization and desinfection - methods of taking blood (vein, fingertip) - using and cleaning of the diluting pipettes - Bürker's chamber - Westergren's tube - centrifuges - blood smear- Principles of: bleeding time blood clotting time Staining index Price-Jones' curve partial thromboplastin time thrombin time

5th week Visit at the Department of Anatomy

6th week 1st MTO (Anatomy, membrane, electrical phenomena)

7th week Holiday

8th week Blood I. Blood tests - hematocrit (micro-, macro-) - determination of hemoglobin concentration (Drabkin) - erythrocyte sedimentation rate by Westergren - differential count (leukocytes) - osmotic resistance of red blood cells - prothrombin time - blood groups (AB0, Rh) - red blood cell count - white blood cell count - thrombocyte count (Fischer-Germer) - reticulocyte count

9th week Blood II. (see above)

10th week 2nd MTO (transmission, transmitters, autonomic nervous system, muscle, blood, fluid compartments)

11th week Studies of the circulatory system I - video - in situ registration of the activity of the heart in the frog - effects of electrical stimulation of the heart - effects of thermal stimulation of the heart - Stannius' ligatures - summation - all or none law - Goltz reflex

12th week Studies of the circulatory system II - video - circulation in the frog tongue - the lymphatic heart of the frog - isolated and surviving frog's heart preparations - effects of ions (adrenaline, acetylcholine, atropine) on the heart - study of the peripheral circulation in the frog (Laewan-Trendelenburg) - experiments in the isolated rat heart preparation (Langendorf perfusion)

13th week The human circulatory and respiratory system - ECG - peripheral pulse, characteristics of the radial pulse - palpation over the chest - auscultation over the heart - the effects of physical exercise on circulation - spirometry - determination of inspiratory and expiratiory pressures - effects of breathing on the circulation - cold pressor test - blood pressure measurement

14th week 3rd MTO (heart, circulation)

15th week Repetition